Oregon Health & Science University

Senior Research Assistant

Job Locations US-OR-Portland
Requisition ID
Position Category
Position Type
Regular Full-Time
Job Type
Molecular Microbiology and Immunology
Salary Range
$39,534 - $49,479 per year, commensurate with qualification, experience and equity.
Monday - Friday, some weekends
Variable, 8:00am - 5:00pm
HR Mission
School of Medicine
Drug Testable

Department Overview

The Senior Research Assistant (SRA) in the Nakai lab will participate in the AAV vector and gene therapy projects conducted by the laboratory. The lab's major research focus is to elucidate the AAV vector-animal host interactions using contemporary high-throughput molecular biology technologies and develop novel AAV vectors for broader applications of this vector system to treating various human diseases.

Function/Duties of Position

The responsibilities of SRA include overseeing AAV vector construction and production, conducting molecular and cellular biology experiments that require advanced knowledge and skillsets and substantial wet lab benchwork experience, overseeing the lab projects and safety together with the PI and doctoral fellows, and managing the laboratory in various aspects. The management work includes inventory and ordering of lab supplies, managing and maintenance of radioisopes, training of new lab personnel and students, scheduling the lab meetings, and maintaining and updating the lab websites. These responsibilities will be assigned to SRA in addition to the daily wet lab bench work outlined below as well as, performing quality control experiments and various types of downstream analyses including Illumina sequencing. Duties will include:

  • Perform various molecular and cellular biology experiments: The work includes but is not limited to designing experiments together with the PI and doctoral fellows, construction and preparation of plasmid DNA, production and purification of AAV vectors, performing tissue culture cell experiments and animal experiments, extraction of DNA / RNA from samples, preparing Sanger/Illumina sequencing samples and their data analysis.
  • Lab Management: This includes the oversight of the daily operation of the lab including the safety monitoring and the experiments performed by lab members, troubleshooting problems lab members may come across, inventory and purchasing of lab supplies including radioisotopes, monitoring the functions of the lab equipment and the cleanliness of the lab, and the oversight of the timely washing and autoclaving of dishes, beakers and flasks, as well as the timely disposal of hazardous waste. The work also includes management of the lab website.
  • Collect, record, organize and verifiy the accuracy of data. Conduct literature searches and summarize information in an appropriate format for a particular study; document and interpret results of experiments and report to principal investigator. Present experimental results at lab meetings and participate in departmental and group seminars. Prepare necessary documents to help the PI write grants and scientific papers.

Required Qualifications

  • Bachelor's Degree with major courses in field of research and 1 to 3 years of relevant experience
  • Research experience in a molecular biology lab
  • Advanced skillsets of various molecular biology technologies including plasmid DNA cloning, DNA ligation and Gibson assembly, plasmid preparations, PCR, qPCR, DNA/RNA extraction, western blotting
  • Ability to work autonomously and coordinate multiple tasks simultaneously
  • Ability to organize a large number of samples and perform high-throughput sample processing correctly and effectively

Preferred Qualifications

  • Master’s Degree
  • Experience with mammalian cell culture experiments
  • Experience with the next-generation sequencing sample preparation
  • Experience with immunostaining and immunofluorescence microscopy

Additional Details

Apply here online. Please be sure to upload a cover letter and resume or CV.

All are welcome

Oregon Health & Science University values a diverse and culturally competent workforce. We are proud of our commitment to being an equal opportunity, affirmative action organization that does not discriminate against applicants on the basis of any protected class status, including disability status and protected veteran status. Individuals with diverse backgrounds and those who promote diversity and a culture of inclusion are encouraged to apply. To request reasonable accommodation contact the Affirmative Action and Equal Opportunity Department at 503-494-5148 or aaeo@ohsu.edu.

As an organization devoted to the health and well-being of people in Oregon and beyond, OHSU requires its employees to be fully vaccinated against COVID-19.


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