Research focused on understanding how herpes simplex virus (HSV) proteins and virus particles are transported in neuronal axons. The position will involve manipulating and producing plasmid DNAs, which will be transfected into neurons cells to produce HSV proteins that are transported into neuronal axons.  Other work will involve studies of protein-protein interactions to understand which HSV proteins interact with kinesin motors that transport virus particles in axons.  The position requires that the candidate have experience with molecular biology: characterizing proteins using polyacrylamide gels and western blots, manipulating plasmid DNAs, cell culture and transfection of cells.  Laboratory experience with manipulating and expressing plasmids and with mammalian cell culture and transfection of cells is essential.  Experience with handling viruses such as HSV or other viruses will also be valuable.  Experience with culturing neurons will also be very helpful. Applicants must have a BSc, although those with MSc or PhD degrees and experience will be given priority.

• Manipulating and producing plasmid DNAs, which will be transfected into neurons to characterize protein transport into axons.
• Producing virus stocks and using these viruses to infect neurons
• Passaging and caring for neuronal cell lines and imaging these cells using confocal microscopy in order to determine how viruses and viral proteins are transported into neuronal axons
• Supervising an RA1 who will order supplies, wash dishes, do routine maintenance in the lab and perform experiments.
• Preparing representations of data and working with the PI to write scientific papers and write grant applications.  

 

• Bachelor's in relevant field AND 1 years of relevant experience OR
• Equivalent combination of training and experience
• Laboratory experience with manipulating and expressing plasmids and with mammalian cell culture and transfection of cells is essential.  

• Experience with transfecting cells, propagating viruses and culturing neurons will also be very valuable. 

Work Schedule:

 

Full time days, location RJH, standard microbiology laboratory conditions, exposures such as hazardous chemicals and biohazard waste. Will perform common laboratory protocols and tissue culture work.

Oregon Health & Science University values a diverse and culturally competent workforce. We are proud of our commitment to being an equal opportunity, affirmative action organization that does not discriminate against applicants on the basis of any protected class status, including disability status and protected veteran status. Individuals with diverse backgrounds and those who promote diversity and a culture of inclusion are encouraged to apply. To request reasonable accommodation contact the Affirmative Action and Equal Opportunity Department at 503-494-5148 or aaeo@ohsu.edu.


As an organization devoted to the health and well-being of people in Oregon and beyond, OHSU requires its employees to be fully vaccinated against COVID-19.