This role is a key position as a research assistant in the laboratory of Dr. Brian O'Roak studying the genetic basis and developmental neuroscience of autism and related disorders. Key responsibilities will include supporting studies utilizing induced pluripotent stem cells (iPSCs), human cell culture, rodent studies, as well as human genomics projects. Applicants must have basic laboratory skills and knowledge of standard molecular biology and cell culturing techniques, such as DNA extraction, molecular cloning, PCR, Sanger sequencing, working in tissue culture. A strong background with stem cell tissue culture and/or rodent models, including genotyping and animal husbandry, is strongly preferred. Experience in neuroscience, human genetics/genomics, or molecular biology (genome editing) is also preferred, as well as a bachelor’s degree in the biological sciences. This position demands excellent organizational skills, time flexibility, and the ability to work both independently and in a team environment.

• Plans, conducts, and completes laboratory experiments under the supervision of the head of the laboratory. Specific duties will the culture of induced pluripotent stem cells (iPSCs) and other cell types.
• Provides assistance with mouse colony management and husbandry, tissue dissection, and genotyping. 
• Performs primary molecular genetic techniques including PCR, gel electrophoresis, enzymatic analyses, fluorescence assisted cell sorting (FACS), fluorescent and light microscopy, plasmid preparation, DNA isolation from mouse and human tissues, next-generation library preparation, and studies of gene expression. 
• Assignments require the performance of complex test procedures and analyses involving multiple procedures and a variety of technical approaches. Operates a variety of instruments and scientific equipment, including operation, quality control and maintenance. Assists with experiments and assays, preparing solutions, collecting and entering data, maintaining databases, assuring compliance with established safety procedures and maintaining required documentation.
• Collects, records, organizes and verifies the accuracy of data. Conducts literature searches and summarizes information in an appropriate format for a particular study; Documents and interprets results of experiments and reports to principal investigator. Presents experimental results at lab meetings and participates in journal and research group meetings.  
• Assists in the daily operation of the lab including hazardous waste disposal, sterilization of equipment, cleaning glassware, general servicing of equipment and ordering lab supplies, and preparation of media and buffers. 

• Bachelor's in relevant field; OR
• Associate's AND 3 years of relevant experience; OR
• 4 years of relevant experience; OR
• Equivalent combination of training and experience
• Experience in an academic research setting, especially with qualitative and/or quantitative methods of data collection
• Basic laboratory skills and knowledge of standard molecular biology techniques
• Sterile tissue culture technique
• High level of attention to detail and proven ability to manage time effectively, especially with competing priorities
• Ability to communicate well and work with faculty and staff within the department and University
• Ability to take initiative and work independently and in a team environment
• Ability to complete tasks in an accurate and timely manner

• Bachelor’s degree in the biological sciences

• 2-4 years of experience serving on a research team

• Pluripotent stem cell culture

• Mouse handling and colony management 

• Human genetics/genomics

• Advanced molecular biology

• Next-generation library preparation

Oregon Health & Science University values a diverse and culturally competent workforce. We are proud of our commitment to being an equal opportunity, affirmative action organization that does not discriminate against applicants on the basis of any protected class status, including disability status and protected veteran status. Individuals with diverse backgrounds and those who promote diversity and a culture of inclusion are encouraged to apply. To request reasonable accommodation contact the Affirmative Action and Equal Opportunity Department at 503-494-5148 or aaeo@ohsu.edu.